Центр молекулярних і клітинних досліджень
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Центр молекулярних і клітинних досліджень створено 2004 року.
Стратегічна мета діяльності Центру - сформувати школу клітинної біології та біотехнології, що дасть змогу готувати висококваліфікованих кадрів як для викладання у вищих навчальних закладах, так і для науково-дослідних установ та медико-біологічних центрів, діяльність яких пов'язана з клітинною біологією і спрямована на вирішення проблем, пов'язаних із аналізом, накопиченням і оцінкою клітинних популяцій як трансплантатів.
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Item Comparative mathematical analysis of functional properties of mice bone marrow in the phase of recovery of colony-forming units number after sub-lethal and repeated sub-lethal irradiation(2020) Boiko, Roman; Bilko, Denys; Russu, Iryna; Bilko, NadiiaBasing on the analysis of experimental data, reported in [N.V. Butomo et al. Radiobiologiya 28 (1988) 39], by applying original mathematical model, earlier and more intensive recovery of the number of colony-forming units of mice bone marrow was shown after repeated sub-lethal irradiation in the dose of 2.75 Gy, comparing to the recovery process of bone marrow colony-forming units after the first sub-lethal irradiation in the dose of 2.75 Gy.Item Control of the genotoxicity level of certain mycotoxins by a fiber optical SOS-type biosensor(2018) Starodub, N.; Fedelesh-Morenetz, M.; Savchuk, M.; Taran, O.; Naboka, O.; Shuliak, L.In addition to their well-known general toxicity, mycotoxins have also been found to often exert genotoxicity. In turn, not only the presence of the mycotoxins is required to be determined, but such DNA-damaging effects by these substances are also needed to be detected in routine monitoring of mycotoxin. The main purpose of this article is to demonstrate the utility of a proposed special fiber optical SOS-chromotest type biosensor, based on the SOS-lux assay in recombinant Escherichia coli cells, in rapid measurement of the genotoxicity level of mycotoxins. The optrode-based fiber optic biosensor and its main operating conditions for such express analysis have been worked out, moreover, its efficacy in the determination of the effects of certain investigated mycotoxins e.g.,T2 mycotoxin, patulin, aflatoxin B2, zearalenone and ochratoxin on the genetic apparatus applied and their activation of the operon of the reparative genes is reported. The genotoxicity of the mycotoxins studied fell in the order: T2 mycotoxin > aflatoxin B2 > patulin >> ochratoxin > zearalenone.Item Detection of air-borne mycotoxin levels by immunobiosensor(2018) Starodub, Nickolaj; Kluchevich, M.; Székács, A.; Vigera, S.The main purpose of this article is to investigate the possibility of detection of the level of mycotoxins in air samples by a surface plasmon resonance (SPR) immunobiosensor. Air samples of 0.25-0.5 m3 volume collected using a special miniature air pump device were passed through 20% acetonitrile as an absorption liquid, and 20 μl aliquots of the extract were analyzed by an SPR immunobiosensor. Comparative analyses by the determination of T2 mycotoxin content were carried out in air and in grain samples upon dispersion in a phytotron. Moreover, mycotoxin content was investigated also under field conditions in air and in vegetable matrix, in the absence and presence of Fusarium fungi, and results were shown to have similar characteristics: the level of mycotoxins detected in air samples correlated with that in grain samples. Thus, results of air-borne mycotoxin detection were correlate with mycotoxin levels detected in environmental samples and may used for screening purposes.Item Detection of biogenic polyamines in blood of patients with breast cancer(2019) Prylutskyi, Maksym; Bilko, Nadiia; Starodub, NickolajThe main threat of cancer diseases is their spreading throughout the population of many countries of the world and the complexity of their diagnostics at the early stages. Because of that, search and development of the latest diagnostic methods for oncological diseases which would allow them to be diagnosed more precisely and rapidly is going on. The objective of the study was developing a method for qualitative and quantitative analysis of polyamines as potential tumour markers in blood serum of patients with breast cancer using a newly created immune biosensor based on the effect of surface plasmon resonance (SPR). In the process of the study we used basics of immune analysis, methods of biosensor analysis with preliminary modification of biosensor analytical surface with certain reagents, such as protein A and BSA, for better orientation of the sensitive layer made from antibodies. During the study 30 samples of blood serum were analyzed, 21 of which were obtained from the patients with breast cancer and 9 samples were normal, taken from healthy people and used as controls. Analysis of blood serum samples was made, using previously created calibration curve, based on polyamine solutions in concentrations from 5 ng/mL to 1 µg/mL. It allowed determination of the presence of polyamines in blood samples and approximate concentration of polyamines comparing resonance angle shift in calibration curve and blood samples. According to the obtained results, the concentration of polyamines exceeded their physiological levels and was in the range of 21.3–125.1 ng/mL. The proposed approach allows one to determine the presence and approximate concentrations of polyamines in range from 5 ng/mL to 1 µg/mL in samples of blood serum of patients with breast cancer which correlates with tumour size and the age of the patients.Item Development of a dietary supplement for improving the cognitive and lowering the homocysteine level(2022) Romaniuk, Svitlana; Tykhonenko, Tetiana; Siromolot, Andriy; Guzyk, Mykhailo; Lugovska, Natalia; Galkin, Olexander; Kuchmerovska, Tamara; Kolybo, Denys; Komisarenko, SergiyPurpose. To develop the dietary supplement for reducing high homocysteine levels, which has a minimum content of components that may cause side effects; to test the supplement effect on the cognitive abilities of ani-mals and to commercialize the product.Materials and Methods. The developed dietary supplement Alfacognitin contains vitamins B6, B9, B12, C, and choline. For modelling experimental hyperhomocysteinemia in rats, the animals are kept on a diet rich in L-methionine. Blood homocysteine concentrations are determined by the ion exchange liquid column chromato-graphy method with the use of an automatic amino acid analyzer. The behavioral responses and cognitive abilities of the rats have been studied with the use of behavioral tests (open field test, fear conditioning test, and social int e raction test). The production of Alfacognitin dietary supplement has been launched with Nutrimed Ltd. (Kyiv).Item Effects of fetal stem cells on life quality of patients with ulcerative colitis(2018) Demchuk, Mariya; Ivankova, Olena; Klunnyk, Mariya; Matiyashchuk, Iryna; Sych, Nataliia; Sinelnyk, Andriy; Karayev, T.; Shulak, M.; Skalozub, Marina; Sorochynska, KhrystynaIn this article we briefly summarized on the main characteristic features of ulcerative colitis (UC) and accentuated on reduced life quality among the patients with UC, in particular, effect of clinical manifestations of the disease on everyday life was described in such patients. Unambiguously, the principal direction to better life quality in patients with UC consists in decreasing activity of inflammatory process. Significant aspect in solution of this problem for present day suggests use of fetal stem cells (FSCs) in complex treatment of the patients suffering from UC. Specifically, by virtue of stem cell therapy use, rapid lowering of clinical signs of UC was recorded, patients revealed stabilization of hemoglobin and total protein levels in blood. Changes of such parameters in the CG patients tended to be much slower. Within the whole period under study, we recorded a consistent pattern between decreased evidence of underlying disease manifestation that is coupled with higher life quality among the UC patients. In conformity with IBDQ international questionnaire, describing that bowel symptoms, systemic symptoms, emotional and social function, life quality among UC patients of the MG were increased (51.33% vs. 96.43%), whereas the patients of the CG reported the value 61.16% that was higher only within the range of 86.16%. Administration of suspensions with FCSs for complex therapy in UC patients proved to be one of the ways for refining social and psychological wellbeing of the individuals under study.Item Epitopes identification of broadly neutralizing monoclonal antibodies against Corynebacterium diphtheriae exotoxin(2022) Kulyk, Ya.; Siromolot, Andriy; Kolybo, DenysBetter and high-potency vaccines against diphtheria are urgently needed to provide broader protection against diverse strains and subtypes. Identification of novel broadly neutralizing epitopes targeted by protective antibodies could aid in such efforts. Aim. In this study we focused on the search of binding sites identification of anti diphtheria toxin monoclonal antibodies and their neutralizing activity to block binding of recombinant exotoxin derivates with host receptors.Item Evaluation of Efficiency of SPR-Based Biosensor and ZnO Photoluminescent Biosensor ib the Anlysys of Biogenic Polyamines(2020) Prylutskyi, Maksym; Starodub, Nickolaj; Taran, O.In this research it was demonstrated the efficiency of the biosensors based on the different principles at the detection of the biogenic amines which are as informative elements at the diagnostic of the cancer events including development of the breast cancer. It was compared two type of the optical devices namely based on the surface plasmon resonance (SPR) and on the photoluminescence of the ZnO nanostructures. Both types of the biosensors could able to detect such low molecular agents as polyamines in model solution at the concentration from 10 to 100 ng/ml. Nevertheless SPR-based biosensor showed better results than ZnO biosensor at the detecting as low and high concentrations of polyamines. In particular, the differences in the analyse efficiency at the determination of the above indicated concentrations between the used biosensors was in range from 14% to 30%. In spite of this data it was demonstrated that ZnO biosensor also may be used for estimation of level of polyamines at the cancer diagnostics.Item Polyamines as new potential biomarkers for differential diagnosis of prostate cancer and estimation of its aggressiveness(2022) Zaletok, Sofia; Klenov, Oleg; Bentrad, Veronica; Prylutskyi, Maksym; Vitruk, Y. ; Stakhovskyy, E. ; Timoshenko, A.Prostate cancer (PCa) is one of the most common malignancies in older men. The study of tissue markers of PCa can provide information about the state of proliferation and apoptosis in tumors, the susceptibility of tumor cells to metastasis and the mechanisms of resistance to therapy, which, in turn, can help predict the course of the disease and develop personalized treatment. Polyamines (PAs) spermine, spermidine, putrescine are of particular interest in terms of PCa tissue markers. Aim: To investigate the levels of basic and acetylated forms of PAs in the postoperative samples of malignant and benign tumors of the human prostate and evaluate the possibility of their use for differential diagnosis and assessment of the PCa aggressiveness. Object and Methods: 57 postoperative tumor samples from patients with prostate adenocarcinoma of different Gleason score (GS) and clinical stage (T1–T4) and 20 samples of tumors from patients with benign prostate hyperplasia (BPH) were studied. The content of PAs was determined by high performance liquid chromatography.Item Treatment Options for Developmental Delay in Children with Perinatal Damage of Nervous System(2018) Sych, Nataliia; Klunnyk, Mariya; Demchuk, Mariya; Ivankova, Olena; Matiyashchuk, Iryna; Sinelnyk, Andriy; Skalozub, Marina; Sorochynska, KhrystynaObjective: Improvement of treatment in children with developmental delay (DD) resulting from perinatal affection of CNS using combination with conventional therapy and administration of fetal stem cells (FSCs) in suspensions containing stem cells extracted from human fetal liver and brain. Materials and methods: Our study allocated 14 children diagnosed with developmental delay and perinatal damage of CNS including 4 boys aged 1.5 to 14 years, with their mean age 4.23 ± 0.31 yrs. and 3 girls - from 3.5 to 13 yrs (mean age – 3.92 ± 0.21 yrs.) (Allocated to the MG) where along with conventional therapy were administered fetal stem cells. Control group (CG) included 7 patients with Developmental delay (DD) – children treated with conventional methods only, including 4 males (aged 3 to 14 yrs. and average age – 4.11 ± 0.14 yrs.) and 3 females (aged from 3 to 15 yrs. and mean age 4.02 ± 0.15 yrs.). Results: Our research revealed that fetal stem cell therapy, irrespective of developmental delay severity in children with CNS damage during perinatal period, can favorably influence the course of development and their immunological markers in particular. Procedure of fetal stem cell administration is simple and noninvasive (no potential causes of complications or allergy reactions in the patients). To promote substantial effects of fetal stem cells we additionally recommended a complex treatment with medicines; different supplementary methods were used for the patients. Aspects of fetal stem cells effect in children with developmental delay are not fully understood and do need a thorough scientific research. In particular, future in-depth study is demanded, as well as the larger randomized and placebo-controlled trials to identify a potential of fetal stem cellsrelated advantages in children with developmental delay (DD). Conclusion: Fetal stem cells (FSCs) use in complex treatment of patients with developmental delay tends to improve cognitive functions along with motor development of children with nervous system affection; such patients also revealed increased immunology findings.