Determination of the Concentration of Polyamines with SPR-based Immune Biosensor for Early Diagnostics of Breast Cancer
Loading...
Date
2016
Authors
Prylutskyi, Maksym
Starodub, Nickolaj
Bilko, Nadiia
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. The method can detect the studied polyamines spermine and spermidine in concentration less than ’0 ng in ’ mL, and with increasing concentrations a statistical probability of the analysis' result rise sharply. Moreover, the dependence of sensitivity of biosensor response from the concentration of polyamines is in the range of ’0-’00 ng /mL.
Description
Keywords
Polyamines, Antibody, Antigen, Surface plasmon resonance, Breast cancer
Citation
Prylutskyi M. Determination of the Concentration of Polyamines with SPR-based Immune Biosensor for Early Diagnostics of Breast Cancer [electronic resource] / Prylutskyi M., Starodub N., Bilko N. // Journal of Biosensors & Bioelectronics. - 2016. - Vol. 7, Issue 4. - 7 p. - Метод доступу: Інтернет. - DOI: 10.4172/2155-6210.1000227 (переглянуто 26 травня 2017 р.). - Назва з екрана.