005. Факультет природничих наук
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- Item4-(N-Boc-амино)-1Н-1,2,3-триазолкарботиоамиды в синтезе производных новой гетероциклической системы [1,2,3]триазоло[4,5-e][1,4]тиазепина(2021) Сирота, Наталия; Кемский, Сергей; Больбут, Андрей; Чернобаев, Игорь; Лявинец, Александр; Вовк, Михаил4-(N-Boc-амино)-1Н-1,2,3-триазолкарботиоамиды, полученные последовательной обработкой 4-(N-Boc-амино)-1Н-1,2,3-три- азолов n-BuLi и алкилизотиоцианатами при –78÷–60°C, при действии этилбромацетата образуют соответствующие 4-(N-Boc- амино)-5-тиоимидаты, которые в насыщенном НCl диоксане подвергаются внутримолекулярной циклоконденсации в 8-(алкил- имино)-4,8-дигидро-1Н-[1,2,3]триазоло[4,5-e][1,4]тиазепин-5(6Н)-оны.
- Item6-gene promoter methylation assay is potentially applicable for prostate cancer clinical staging based on urine collection following prostatic massage(2019) Nekrasov, Kostyantyn; Vikarchuk, Mark; Rudenko, Evgeniya; Ivanitskiy, Igor; Grygorenko, Viacheslav; Danylets, Rostyslav; Kondratov, A.; Stoliar, Liubov; Sharopov, Bizhan; Kashuba, VolodymyrThe detection of prostate cancer (PCa) biomarkers in bodily fluids, a process known as liquid biopsy, is a promising approach and particularly beneficial when performed in urine samples due to their maximal non‑invasiveness requirement of collection. A number of gene panels proposed for this purpose have allowed discrimination between disease‑free prostate and PCa; however, they bear no significant prognostic value. With the purpose to develop a gene panel for PCa diagnosis and prognosis, the methylation status of 17 cancer‑associated genes were analyzed in urine cell‑free DNA obtained from 31 patients with PCa and 33 control individuals using methylation‑specific polymerase chain reaction (MSP). Among these, 13 genes indicated the increase in methylation frequency in patients with PCa compared with controls. No prior association has been reported between adenomatosis polyposis coli 2 (APC2), homeobox A9, Wnt family member 7A (WNT7A) and N‑Myc downstream‑regulated gene 4 protein genes with PCa. The 6‑gene panel consisting of APC2, cadherin 1, forkhead box P1, leucine rich repeat containing 3B, WNT7A and zinc family protein of the cerebellum 4 was subsequently developed providing PCa detection with 78% sensitivity and 100% specificity. The number of genes methylated (NGM) value introduced for this panel was indicated to rise monotonically from 0.27 in control individuals to 4.6 and 4.25 in patients with highly developed and metastatic T2/T3 stage cancer, respectively. Therefore, the approach of defining the NGM value may not only allow for the detection of PCa, but also provide a rough evaluation of tumor malignancy and metastatic potential by non‑invasive MSP analysis of urine samples.
- ItemA new nanocomposite amperometric biosensor for L-lactate determination(2021) Vorobiienko, А.; Biloivan, O.The development of nanocomposite biosensor for l-lactate determination in sweat. The investigation included the development of a high-performance nanocomposite sensor.
- ItemAb initio розрахунки борофосфідних кластерів(2000) Ісаєв, С.; Гребешок, А.; Черногоренко, В.; Корнілов, М.Методом ab initio (HF, МР2) розраховані величини загальної енергії кластерів . для m = = 4 —12. Знайдені ентальпії перетворення моноелементних кластерів у мішані. З'ясовано, що ймовірне утворення кластерів з приблизно однаковою кількістю атомів бору та фосфору. Вже для т = 12 мішані кластери стають невигідними.
- ItemActual Problems of Health Protection of Ukrainian Youth(2015) Posudin, YuriyРозглядаються основні проблеми зі здоров'ям української молоді, викликані неякісним швидким харчуванням, пивним алкоголізмом, курінням. Внаслідок цих згубних звичок, а також непродуманої державної політики та впливу реклами, молоде покоління страждає від низки небезпечних захворювань, що ведуть у ряді випадків до смертельних наслідків.
- ItemAdsorption of proteins on nano-silica modified with antimicrobial drugs(2019) Gaidai, Alina; Vakuliuk, Polina; Furtat, Iryna; Kunytsia, Natalia; Murlanova, Tatiana; Golub, AlexanderМатеріали конференції "Українська конференція з міжнародною участю "Хімія, фізика та технологія поверхні" та практикум "Біосумісні наночастинки на основі металів: синтез та застосування".
- ItemAllelopathic activity analysis of the invasive grassland species Elaeagnus angustifolia L. by t he method of Neubauer & Schneider(2016) Norenko, KaterynaThe material of the speech at 13th Eurasian Grassland Conference Management and Conservation of Semi-natural grasslands: from theory to practice (Sighişoara, Romania, 20-24 September 2016).
- ItemAnalysis and assessment of the environmental situation within Muromets Island (Kyiv)(2022) Derevska, Kateryna; Nesterovskyi, Victor; Manyuk, Volodymyr; Rudenko, Kseniia; Hurina, MariaThe environmental situation within Muromets floodplain island, which is considered one of the oldest and largest islands of the Kyiv metropolis was studied. This sandy island was formed due to fluvial migration and numerous alluvial deposits since the Holocene. The island is located in the modern Dnipro floodplain, bordering Trukhaniv Island from the south. The environmental state of the island’s plants, landscapes, water bodies, soils and rocks was analyzed. The environmental protection objects of the territory were also characterized. Satellite images of the Earth’s surface over the past 37 years were analyzed to study modern changes in the island outline and hydrography within the Dnipro floodplain near Kyiv. Changes in the shoreline of Muromets Island were assessed. Using our field research data, cartographic schemes, space photographs and scientific publications of various years, we characterized the island’s geological environment, presented the dynamics of shoreline development, analyzed the ecological situation, and identified environmental problems. We found that the island’s environmental condition correlates with the influence of both natural and anthropogenic factors. The degree and severity of impact of the above-mentioned factors on the island’s geological environment was established. It was shown that intense changes in this territory occur due to the geological work of surface water and the work of the wind ,which are intensified by anthropogenic actions. The land shoreline has been migrating for a long time as a result of active water erosion by the Dnipro, Desna and Chartoryi (Desenka) rivers and their tributaries. New straits, peninsulas and individual small islands are formed. The results indicate significant natural dynamics of the Dnipro floodplain development, which occurs against the background of anthropogenic influence, the pressure of which is constantly increasing. The complex of anthropogenic and natural factors lead to the active development of invasive plants, eutrophication of water bodies, shoreline erosion, and significant loss of water and land resources of Muromets Island. The conclusions substantiated in the article are of general geological, ecological and practical significance. They are the beginning of environmental monitoring on this issue, expand knowledge about the peculiarities of the geological structure of Kyiv and the Dnipro floodplain, and update data on the development and ecological situation of this territory. This provides an opportunity to predict and determine the direction of development of negative environmental changes in advance, to make early decisions regarding agents of negative influence and pressure on the geological environment, and to respond in time to their localization or termination. The obtained data can also be used in the arrangement of geological and engineering works related to the preservation of the Dnipro floodplain and the shoreline of the dry land, as well as in the planning of environmental protection measures.
- ItemAnalysis of Arabidopsis non-reference accessions reveals high diversity of metabolic gene clusters and discovers new candidate cluster members(2023) Marszalek-Zenczak, Malgorzata; Satyr, Anastasiia; Wojciechowski, Pawel; Zenczak, Michal; Sobieszczanska, Paula; Brzezinski, Krzysztof; Iefimenko, Tetiana; Figlerowicz, Marek; Zmienko, AgnieszkaMetabolic gene clusters (MGCs) are groups of genes involved in a common biosynthetic pathway. They are frequently formed in dynamic chromosomal regions, which may lead to intraspecies variation and cause phenotypic diversity. We examined copy number variations (CNVs) in four Arabidopsis thaliana MGCs in over one thousand accessions with experimental and bioinformatic approaches. Tirucalladienol and marneral gene clusters showed little variation, and the latter was fixed in the population. Thalianol and especially arabidiol/baruol gene clusters displayed substantial diversity. The compact version of the thalianol gene cluster was predominant and more conserved than the noncontiguous version. In the arabidiol/baruol cluster, we found a large genomic insertion containing divergent duplicates of the CYP705A2 and BARS1 genes. The BARS1 paralog, which we named BARS2, encoded a novel oxidosqualene synthase. The expression of the entire arabidiol/baruol gene cluster was altered in the accessions with the duplication. Moreover, they presented different root growth dynamics and were associated with warmer climates compared to the reference-like accessions. In the entire genome, paired genes encoding terpene synthases and cytochrome P450 oxidases were more variable than their nonpaired counterparts. Our study highlights the role of dynamically evolving MGCs in plant adaptation and phenotypic diversity.
- ItemAnalysis of grassland ANPP dynamics due to changes in climate variables at Ukrainian Biosphere Reserve "Askania-Nova"(2017) Belyakov, Sergiy; Gofman, Orysia; Vyshenska, Iryna; Zvegintsev, SergeyThe Ukrainian feather-grass steppe ecosystems are highly vulnerable to climate changes. To study the impact of climatic factors on steppe ecosystems’ productivity, the correlation and stepwise regression analysis between ANPP and other variables were provided. The correlation of bioclimatic variables (month precipitation, relative humidity and air and soil temperatures) and aboveground net primary production (ANPP) were investigated for three study plots that represent major steppe microrelief: plain, slope and lowland. The results of multiple regression analysis showed the major components that influenced the ANPP at each of the study plots "Plain", "Slope" and "Lowland". The precipitation and relative humidity in the months before the vegetation peak were most important for ANPP accumulation. Results of this study are important for the prediction of ecosystem changes under the climate changes and also for the development of nature conservation programmes.
- ItemAnalysis of Moon's gravitational - wave and Earth's global temperature: influence of timetrend and cyclic change of distance from Moon(2018) Matsuki, Yoshio; Bidyuk, PetroThis research examined the influence of Moon’s gravitational-wave to Earth’s global warming process and the effects of time-trend and cyclic change of the distance between Moon and Earth. In the pervious research, we found that the Moon’s gravitational-wave could influence the process of the Earth’s global warming; and, we also found that Moon’s cyclic movement around Earth needed to be further investigated, because it gave a unique pattern of distribution in the data for the empirical analysis; while both global temperature and global carbon-dioxide increase almost linearly in the time-series. In this research we added dummy binary variables that simulate the trend of time and the cyclic changes. As a result we confirmed that the influence of Moon’s gravitational-wave is significant in the process of rising global temperature on Earth.
- ItemAnalysis of polyamine levels in blood serum of patients with breast cancer using optical SPR-based immune biosensor(2019) Prylutskyi, Maksym; Starodub, NickolajThe aim of the study was to develop a method for qualitative and quantitative analysis of polyamines as potential tumour markers in blood serum of patients diseased with breast cancer with use of newly created immune biosensor based on effect of surface plasmon resonance.
- ItemAnalytical approach for calculating the chemotaxis sensitivity function(2017) Vasilev, AlekseyWe consider the chemotaxis problem for a one-dimensional system. To analyze the interaction of bacteria and an attractant, we use a modified Keller-Segel model, which accounts for the attractant absorption. To describe the system, we use the chemotaxis sensitivity function, which characterizes the nonuniformity of the bacteria distribution. In particular, we investigate how the chemotaxis sensitivity function depends on the concentration of an attractant at the boundary of the system. It is known that, in the system without absorption, the chemotaxis sensitivity function has a bell shape maximum. Here, we show that the attractant absorption and special boundary conditions for bacteria can cause the appearance of an additional maximum in the chemotaxis sensitivity function. The value of this maximum is determined by the intensity of absorption.
- ItemAntarctic Hairgrass Rhizosphere Microbiomes: Microscale Effects Shape Diversity, Structure, and Function(2022) Prekrasna, Ievgeniia; Pavlovska, Mariia; Miryuta, Natalia; Dzhulai, Artem; Dykyi, Evgen; Convey, Peter; Kozeretska, Iryna; Bedernichek, Tymur; Parnikoza, IvanThe rhizosphere microbiome of the native Antarctic hairgrass Deschampsia antarctica from the central maritime Antarctic was investigated using 16S RNA metagenomics and compared to those of the second native Antarctic plant Colobanthus quitensis and closely related temperate D. cespitosa. The rhizosphere microbial communities of D. antarctica and D. cespitosa had high taxon richness, while that of C. quitensis had markedly lower diversity. The majority of bacteria in the rhizosphere communities of the hairgrass were affiliated to Proteobacteria, Bacteroidetes, and Actinobacteria. The rhizosphere of C. quitensis was dominated by Actinobacteria. All microbial communities included high proportions of unique amplicon sequence variants (ASVs) and there was high heterogeneity between samples at the ASV level. The soil parameters examined did not explain this heterogeneity. Bacteria belonging to Actinobacteria, Bacteroidetes, and Proteobacteria were sensitive to fluctuations in the soil surface temperature. The values of the United Soil Surface Temperature Influence Index (UTII, It i) showed that variations in most microbial communities from Galindez Island were associated with microscale variations in temperature. Metabolic predictions in silico using PICRUSt 2.0, based on the taxonomically affiliated part of the microbiomes, showed similarities with the rhizosphere community of D. antarctica in terms of the predicted functional repertoire. The results obtained indicate that these communities are involved in the primary processes of soil development (particularly the degradation of lignin and lignin-derived compounds) in the central maritime Antarctic and may be beneficial for the growth of Antarctic vascular plants. However, due to the limitations associated with interpreting PICRUSt 2.0 outputs, these predictions need to be verified experimentally.
- ItemAntifungal activity of new quinolone and acridone-derivatives(2019) Voloshchuk, N.; Vasylchenko, O.; Kosovska, N.; Bashta, O.; Sorokin, O.; Shyrina, T.; Palchykovska, L.Meeting abstract of the he APS Northeastern Division meeting in State College, Pennsylvania, April 3–5, 2019.
- ItemAssessing the influence of different types of anthropogenic impact on beech forest ecosystems structure in Latorica river basin(2013) Kozak, OlenaThe six habitat types in beech forest zone in Latorica river basin have been studied: 1. Primary beech forests; 2. Modified beech forests; 3. Semi-natural beech forests; 4. Secondary hay meadows; 5. Secondary pasture meadows; 6. Ruderal habitat. Indicator species analysis, biodiversity indices calculations (species richness, evenness, Shennon-Wiener index and Simpson index), ecosystem structure and species composition assessment have been performed.
- ItemAssessment of aridization of climatic conditions on the Crimean Peninsula in summer using ground and satellite data(2022) Boychenko, Svitlana; Kuchma, TetianaClimate vulnerability of the Crimean Peninsula is assessed based on the temperature and precipitation changes for the period 1991-2021 and satellite-derived drought index (VHI) variations for the period 2001-2021. There is an increase of temperature on average by 0.78±0.01 oC per 10 years in summer, and a slight change in precipitation in June and July (within 2±1%). While in August there is a decrease of the precipitation amount by 10-12%. According to analysed data, the most severe drought condition manifestation on the Crimean Peninsula over the last twenty years was observed in 2007, 2017, 2018 and 2020. In the central part of the Crimean peninsula, which belongs to the climatic zone of Steppe Crimea, arid conditions had the greatest manifestation and distribution (zones with extreme and severe drought conditions are observed). Minor areas in the mountainous part of the Crimea also have signs of arid conditions, but for the most part the condition of vegetation is satisfactory in this area. In percentage terms, in 2007 VHI values of extreme and severe drought occupied 57% and 20% of the peninsula’s area accordingly, as well 10% and 29% in 2017, 7% and 22% in 2018 and 11% and 26% in 2020.
- ItemAssessment of hematopoietic stem cells in norma and pathology in cell culture in vitro and in vivo(2019) Bilko, Denys; Sviezhentseva, Ilona; Bilko, NadiiaThe aim of the current study was to to determine the role of erythroid lineage of differentiation side by side with granulocyte-macrophage progenitor cell in the mechanism of resistance for TICs therapy.
- ItemAssessment of Hemopoietic Progenitor Cells in Cell Culture Correlates With Cytogenetic Analysis of Patients with Chronic Myeloid Leukemia : [preprint](2010) Bilko, Nadiia; Diachenko, M.; Borbuliak, Iryna; Dyagil, IrynaAbstracts of the XVIII. Wilsede Meeting "Modern Trends in Human Leukemia and Cancer", June 19-23, 2010.