Факультет природничих наук
Permanent URI for this collection
Browse
Browsing Факультет природничих наук by Subject "article"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
Item Association of alpha-synuclein co-pathology with beta-amyloid and phosphorylated tau levels in Alzheimer's disease(2025) Shpylchyn, Vitalii; Vyniavska, PolinaMisfolded α-synuclein (α-syn) aggregates can be present in the cerebrospinal fluid (CSF) of individuals with Alzheimer’s disease (AD), even in the absence of clinical signs of synucleinopathy. This co-pathology may influence AD progression at the molecular level. Detection of α-synuclein aggregates using seed amplification assay (SAA) enables stratification of AD patients beyond classical biomarkers included in the AT(N) framework. The AT(N) framework allows biological classification of AD based on its core pathological processes: β-amyloid aggregation (A), tau accumulation and hyperphosphorylation (T), and non-specific neurodegeneration (N). This study aimed to explore whether α-syn co-pathology, detected by SAA, is associated with altered concentrations and longitudinal trajectories of CSF β-amyloid 42 (Aβ42) and phosphorylated tau 181 (p-tau181) in the biomarker-defined AD group. Data from A+T+ participants (N = 609) in the Alzheimer’s Disease Neuroimaging Initiative (ADNI) were analysed, using Roche Elecsys electrochemiluminescence immunoassay (ECLIA) and SAA results. Substantial discrepancies between clinical diagnosis and AT profiles were observed. Twenty-nine percent of A+T+ participants were α-synpositive (S+), indicating a high prevalence of α-syn co-pathology in biologically defined AD. Cross-sectional comparisons revealed that S+ individuals had lower baseline Aβ42 concentrations compared to α-synnegative (S−) participants. Linear mixed-effects models (LMEMs) showed a significantly steeper decline in Aβ42 over time in the S+ group, supporting the hypothesis that misfolded α-syn aggregation accelerates amyloid aggregation. However, p-tau181 levels increased more slowly in S+ than in S− individuals, contrary to expectations. These associations remained significant after adjustment for age, sex, diagnosis, and APOE-ε4 genotype. These findings suggest that α-syn co-pathology may affect AD progression through its interaction with Aβ42 and support its integration into biomarker-based classification frameworks.Item Long non-coding RNA SNHG1 as a diagnostic and prognostic marker of bladder cance(2025) Bratyshchenko, Anastasiia; Haviaz, Volodymyr; Honcharenko, Anton; Stakhovsky, Eduard; Kononenko, Oleksiy; Rosohatska, Inna; Pasichnyk, Tetiana; Mankovska, OksanaBladder cancer (BC) is one of the most prevalent cancers globally, and it ranks as the fifth most common malignancy among men in Ukraine. Modern diagnostic tools for bladder cancer have significant limitations: some of them are overly invasive and others lack sufficient sensitivity and specificity. Currently, molecular markers are becoming a promising tool for identification of oncogenic processes. Among epigenetic mechanisms involved in malignant cell transformation, long non-coding RNA (lncRNA)s are of particular interest, as they can act as either oncogenes or tumor suppressors. Available studies suggest that the lncRNA SNHG1, which is currently understudied, promotes tumor proliferation and inhibits apoptosis in malignant cells. The aim of the current study was to analyze changes in the relative expression of SNHG1 in tumor tissues and its levels in cell-free urine of bladder cancer patients, and to evaluate its diagnostic and prognostic value. There was a statistically significant increase in the SNHG1 gene expression level in tumor tissues compared to conditionally normal tissues. Also, a significant decrease in relative levels of SNHG1 transcripts was observed in urine of BC patients compared to healthy individuals. According to the analysis of ROC curves analysis, the chosen marker can identify bladder cancer with high sensitivity and specificity. A positive correlation was also discovered between SNHG1 expression level in tumor tissue and cancer stage. The obtained data support the relevance of further study on lncRNA SNHG1 as a promising diagnostic and prognostic marker of bladder cancer.Item Protein aggregates carry non-genetic memory in bacteria after stresses(2020) Kukharenko, O.; Terzova, Vitaliia; Zubova, G.Protein aggregation is related to the formation of oligomers and aggregates, leading to impaired cellular processes. The protein aggregates formation is associated with pathologies and ageing in eukaryotes whereas in bacteria aggregation causes dramatic changes in growth rate, stress resistance and virulence, and eventually these aggregates play a functional role. Both cellular and environmental factors enhance protein damage via aggregation, nonetheless, upon sublethal doses of proteotoxic environmental stressors, protein aggregates may improve cellular robustness and carry a type of non-genetic memory of the previous stressors through several generations. Emerging data on aggregated proteins, carrying non-genetic (epigenetic) traits, show that protein-based inheritance is known within all three kingdoms of living organisms. This review focuses on the protein aggregates as carriers of non-genetic memory in bacteria.