Том 8

Permanent URI for this collection

https://ekmair.ukma.edu.ua/handle/123456789/36406

Browse

Browsing Том 8 by Subject "article"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Association of alpha-synuclein co-pathology with beta-amyloid and phosphorylated tau levels in Alzheimer's disease
    (2025) Shpylchyn, Vitalii; Vyniavska, Polina
    Misfolded α-synuclein (α-syn) aggregates can be present in the cerebrospinal fluid (CSF) of individuals with Alzheimer’s disease (AD), even in the absence of clinical signs of synucleinopathy. This co-pathology may influence AD progression at the molecular level. Detection of α-synuclein aggregates using seed amplification assay (SAA) enables stratification of AD patients beyond classical biomarkers included in the AT(N) framework. The AT(N) framework allows biological classification of AD based on its core pathological processes: β-amyloid aggregation (A), tau accumulation and hyperphosphorylation (T), and non-specific neurodegeneration (N). This study aimed to explore whether α-syn co-pathology, detected by SAA, is associated with altered concentrations and longitudinal trajectories of CSF β-amyloid 42 (Aβ42) and phosphorylated tau 181 (p-tau181) in the biomarker-defined AD group. Data from A+T+ participants (N = 609) in the Alzheimer’s Disease Neuroimaging Initiative (ADNI) were analysed, using Roche Elecsys electrochemiluminescence immunoassay (ECLIA) and SAA results. Substantial discrepancies between clinical diagnosis and AT profiles were observed. Twenty-nine percent of A+T+ participants were α-synpositive (S+), indicating a high prevalence of α-syn co-pathology in biologically defined AD. Cross-sectional comparisons revealed that S+ individuals had lower baseline Aβ42 concentrations compared to α-synnegative (S−) participants. Linear mixed-effects models (LMEMs) showed a significantly steeper decline in Aβ42 over time in the S+ group, supporting the hypothesis that misfolded α-syn aggregation accelerates amyloid aggregation. However, p-tau181 levels increased more slowly in S+ than in S− individuals, contrary to expectations. These associations remained significant after adjustment for age, sex, diagnosis, and APOE-ε4 genotype. These findings suggest that α-syn co-pathology may affect AD progression through its interaction with Aβ42 and support its integration into biomarker-based classification frameworks.
  • Thumbnail Image
    Item
    Colibacillosis in broiler chicks and its etiological link to the biological characteristics of Escherichia coli isolates from the breeder flock
    (2025) Nechypurenko, Oleksii; Furtat, Iryna; Dreval, Denis; Avdeeva, Liliya
    Pathogenic strains of Escherichia coli, capable of causing avian colibacillosis (APEC), currently pose a significant threat to modern poultry farming, in particular industrial broiler production. Moreover, the origin of colibacillosis in broiler chickens is connected with parental health issues. Therefore, the aim of the work was to investigate the etiological link between colibacillosis in 1-day old chicks and the biological characteristics of E. coli isolates from the breeder flock. A total of 65 pathogenic E. coli isolates were detected from broiler-breeders (n = 14) and 1-day-old chikens (n = 51). This shows a huge impact of collibacillosis on poultry farming at different levels of production. The main lesions were associated with fibrin deposition and omphlaitis formation. The last-mentioned parameter could affect the viability of chicks; nevertheless, the average Pasgar score was identified as 9.7 points. Amomg APEC, the most prevalent were O78 and O18 serotypes, wich were characterized by the absence of β-hemolysis. Moreover, the antibiotic profile of APEC isolated from parenteral and progeny flocks was similar. A high level of resistance to amoxicillin, amoxiclav, doxycycline, tetracycline, and enrofloxacin was found; more than 70% of isolated APEC were invulnerable to the mentioned antimicrobials. The most effective antibiotics were colistin, gentamicin, and florfenicol, to which only 0.0% and 2.0%, 14.3 and 9.8%, 7.1% and 2.0% of isolated APEC from broiler-breeders and chickens, respectively, were resistant. In addition, prolonged treatment of birds led to the formation of multi-resistant strains that affected chickens from the first days of life. Thus, studying the process of APEC infection through an integrated production chain can be useful for taking appropriate measures to prevent early cases of colibacilosis.
  • Thumbnail Image
    Item
    Long non-coding RNA SNHG1 as a diagnostic and prognostic marker of bladder cance
    (2025) Bratyshchenko, Anastasiia; Haviaz, Volodymyr; Honcharenko, Anton; Stakhovsky, Eduard; Kononenko, Oleksiy; Rosohatska, Inna; Pasichnyk, Tetiana; Mankovska, Oksana
    Bladder cancer (BC) is one of the most prevalent cancers globally, and it ranks as the fifth most common malignancy among men in Ukraine. Modern diagnostic tools for bladder cancer have significant limitations: some of them are overly invasive and others lack sufficient sensitivity and specificity. Currently, molecular markers are becoming a promising tool for identification of oncogenic processes. Among epigenetic mechanisms involved in malignant cell transformation, long non-coding RNA (lncRNA)s are of particular interest, as they can act as either oncogenes or tumor suppressors. Available studies suggest that the lncRNA SNHG1, which is currently understudied, promotes tumor proliferation and inhibits apoptosis in malignant cells. The aim of the current study was to analyze changes in the relative expression of SNHG1 in tumor tissues and its levels in cell-free urine of bladder cancer patients, and to evaluate its diagnostic and prognostic value. There was a statistically significant increase in the SNHG1 gene expression level in tumor tissues compared to conditionally normal tissues. Also, a significant decrease in relative levels of SNHG1 transcripts was observed in urine of BC patients compared to healthy individuals. According to the analysis of ROC curves analysis, the chosen marker can identify bladder cancer with high sensitivity and specificity. A positive correlation was also discovered between SNHG1 expression level in tumor tissue and cancer stage. The obtained data support the relevance of further study on lncRNA SNHG1 as a promising diagnostic and prognostic marker of bladder cancer.