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Browsing Інші публікації by Author "Bratyshchenko, Anastasiia"

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    Long non-coding RNA SNHG1 as a diagnostic and prognostic marker of bladder cance
    (2025) Bratyshchenko, Anastasiia; Haviaz, Volodymyr; Honcharenko, Anton; Stakhovsky, Eduard; Kononenko, Oleksiy; Rosohatska, Inna; Pasichnyk, Tetiana; Mankovska, Oksana
    Bladder cancer (BC) is one of the most prevalent cancers globally, and it ranks as the fifth most common malignancy among men in Ukraine. Modern diagnostic tools for bladder cancer have significant limitations: some of them are overly invasive and others lack sufficient sensitivity and specificity. Currently, molecular markers are becoming a promising tool for identification of oncogenic processes. Among epigenetic mechanisms involved in malignant cell transformation, long non-coding RNA (lncRNA)s are of particular interest, as they can act as either oncogenes or tumor suppressors. Available studies suggest that the lncRNA SNHG1, which is currently understudied, promotes tumor proliferation and inhibits apoptosis in malignant cells. The aim of the current study was to analyze changes in the relative expression of SNHG1 in tumor tissues and its levels in cell-free urine of bladder cancer patients, and to evaluate its diagnostic and prognostic value. There was a statistically significant increase in the SNHG1 gene expression level in tumor tissues compared to conditionally normal tissues. Also, a significant decrease in relative levels of SNHG1 transcripts was observed in urine of BC patients compared to healthy individuals. According to the analysis of ROC curves analysis, the chosen marker can identify bladder cancer with high sensitivity and specificity. A positive correlation was also discovered between SNHG1 expression level in tumor tissue and cancer stage. The obtained data support the relevance of further study on lncRNA SNHG1 as a promising diagnostic and prognostic marker of bladder cancer.
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    Non-invasive biomarkers for bladder cancer: a study on lncRNAs and DNA methylation
    (2025) Haviaz, V.; Bratyshchenko, Anastasiia; Skrypnikova, Olena; Kariaka, S.; Honcharenko, A.; Todoryshyn, D.; Khokhliuk, O.; Rosohatska, I.; Kashuba, Volodymyr; Kononenko, O.; Vikarchuk, Mark; Panasenko, G.; Mankovska, Oksana
    Modern diagnostic methods for bladder cancer (BC) have some limitations, including invasiveness and low sensitivity, making the search for new molecular markers important. Aim. To identify potential epigenetic markers for BC diagnosis and progression, including the methylation of gene promoters and the lncRNAs expression levels in tumor tissues and liquid biopsies. Methods. RT-qPCR, MSP. Results. Significant diagnostic value of the VIM, TMEFF2, NKX3.1, MYO3A, GDF15, and RASSF1A methylation in BC patients, increased the RASSF1A expression in tumor tissues and decreased the RASSF1A and ANRASSF1 in liquid biopsies. Correlations were identified between the studied markers and the clinical characteristics of patients. Conclusions. Methylation of the genes VIM, TMEFF2, NKX3.1, MYO3A, GDF15, and RASSF1A, and changes in the expression of the lncRNAs ANRASSF1, PANDAR, and GAS5 may play an important role in BC pathogenesis. The data obtained underscore the relevance of further studies on these markers for BC diagnosis and progression assessment.