Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles

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dc.contributor.authorPrylutskyi, Maksym
dc.contributor.authorStarodub, Mykola
dc.date.accessioned2020-06-10T21:22:55Z
dc.date.available2020-06-10T21:22:55Z
dc.date.issued2019
dc.description.abstractAim: To analyze the performance of biosensor based on nanoparticles of zinc oxide for the detection of spermine and spermidine in solution and in cell culture. Materials and Methods: Zinc oxide nanoparticles were used for preparing biosensor containing antibodies to spermine and spermidine. Polyamine concentration is solutions of spermine and spermidine as well as in lyophilisate of MCF-7 cells was measured by luminescence of the samples excited by laser beam at 380 nm. Results: The minimum concentration for the detection of polyamines in model solutions is 10 ng/ml, and maximum one is 100 ng/ml. A higher level of luminescence intensity of nanoparticles was found during analysis the polyamines in MCF-7 lyophilisate allowing for detecting polyamines at concentrations from 100 cells/ml to 100,000 cells/ml. Conclusions: The proposed biosensor system for determining the level of biogenic polyamines in cell lyophilisate using the optical properties of zinc oxide nanoparticles is promising for further improvement of the methodology and its implementation for detection and measurement of polyamines in biological systems.en_US
dc.identifier.citationPrylutskyi M. P. Detection of polyamines using immunobiosensor based on zinc oxide nanoparticles [electronic resource] / Prylutskyi M. P., Starodub M. F. // Experimental oncology. - 2019. - Vol. 41, Issue 4. - P. 363-365.en_US
dc.identifier.urihttps://doi.org/10.32471/exp-oncology.2312-8852.vol-41-no-4.13920
dc.identifier.urihttps://ekmair.ukma.edu.ua/handle/123456789/17404
dc.language.isoenuk_UA
dc.relation.sourceExperimental oncologyen_US
dc.statusfirst publisheduk_UA
dc.subjectMCF-7 cellsen_US
dc.subjectbiosensorsen_US
dc.subjectphotoluminescenceen_US
dc.subjectnanostructuresen_US
dc.subjectspermineen_US
dc.subjectspermidineen_US
dc.subjectarticleen_US
dc.titleDetection of polyamines using immunobiosensor based on zinc oxide nanoparticlesen_US
dc.typeArticleuk_UA
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Кафедра лабораторної діагностики біологічних систем