Ascorbic Acid-Induced Cardiac Differentiation of Murine Pluripotent Stem Cells : Transcriptional Profiling and Effect of a Small Molecule Synergist of Wnt/β-Catenin Signaling Pathway

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dc.contributor.authorIvanyuka, Dinaen_US
dc.contributor.authorBudasha, Galinaen_US
dc.contributor.authorZhenga, Yunjieen_US
dc.contributor.authorGaspara, John Antonyen_US
dc.contributor.authorChaudhari, Umeshen_US
dc.contributor.authorFatima, Azraen_US
dc.contributor.authorBahmanpour, Soghraen_US
dc.contributor.authorGrin, Vladislav K.en_US
dc.contributor.authorPopandopulo, Andrey G.en_US
dc.contributor.authorSachinidisa, Agapiosen_US
dc.contributor.authorHescheler, Jürgenen_US
dc.contributor.authorSaric, Tomoen_US
dc.date.accessioned2026-03-06T08:44:03Z
dc.date.available2026-03-06T08:44:03Z
dc.date.issued2025
dc.description.abstractBackground: Reproducible and efficient differentiation of pluripotent stem cells (PSCs) to cardiomyocytes (CMs) is essential for their use in regenerative medicine, drug testing and disease modeling. The aim of this study was to evaluate the effect of some previously reported cardiogenic substances on cardiac differentiation of mouse PSCs. Methods: Differentiation was performed by embryoid body (EB)-based method using three different murine PSC lines. The differentiation efficiency was monitored by RT-qPCR, immunocytochemistry and flow cytometry, and the effect mechanistically evaluated by transcriptome analysis of treated EBs. Results: Among the five tested compounds (ascorbic acid, dorsomorphin, cyclic adenosine 3',5'-monophosphate, cardiogenol C, cyclosporin A) only ascorbic acid (AA) exerted a strong and reproducible cardiogenic effect in CGR8 cells which was less consistent in other two PSC lines. AA induced only minor changes in transcriptome of CGR8 cells after administration during the initial two days of differentiation. Cardiospecific genes and transcripts involved in angiogenesis, erythropoiesis and hematopoiesis were up-regulated on day 5 but not on days 2 or 3 of differentiation. The cardiac differentiation efficiency was improved when QS11, a small-molecule synergist of Wnt/p-catenin signaling pathway, was added to cultures after AA-treatment. Conclusion: This study demonstrates that only minor transcriptional changes are sufficient for enhancement of cardiogenesis of murine PSCs by AA and that AA and QS11 exhibit synergistic effects and enhance the efficiency of CM differentiation of murine PSCs.en_US
dc.identifier.citationAscorbic Acid-Induced Cardiac Differentiation of Murine Pluripotent Stem Cells : Transcriptional Profiling and Effect of a Small Molecule Synergist of Wnt/β-Catenin Signaling Pathway / Ivanyuk D., Budash G., Zheng Y. [et al] // Cellular Physiology and Biochemistry. - 2015. - Vol. 36. - P. 810-830. - https://doi.org/10.1159/000430140en_US
dc.identifier.issn1015-8987
dc.identifier.urihttps://doi.org/10.1159/000430140
dc.identifier.urihttps://ekmair.ukma.edu.ua/handle/123456789/38549
dc.language.isoenen_US
dc.relation.sourceCellular Physiology and Biochemistryen_US
dc.statusfirst publisheden_US
dc.subjectPluripotent stem cellsen_US
dc.subjectcardiomyocytesen_US
dc.subjectDifferentiationen_US
dc.subjectTranscriptomeen_US
dc.subjectSmall moleculesen_US
dc.subjectiPSCen_US
dc.subjectESCen_US
dc.subjectSscorbic aciden_US
dc.subjectWnt pathwayen_US
dc.subjectarticleen_US
dc.titleAscorbic Acid-Induced Cardiac Differentiation of Murine Pluripotent Stem Cells : Transcriptional Profiling and Effect of a Small Molecule Synergist of Wnt/β-Catenin Signaling Pathwayen_US
dc.typeArticleen_US
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