The hybridoiogical analysis was provided on several durum wheat genotypes with utilizing three F2 populations developed from the crossing between parental forms that differed in the invariable malt-zone triplet on elecirophoretic spectrum of α -amylase. Three components of this zone are controlled by three genes with an independent way of inheritance: one of them is located on the 6B or 5B chromosome, and two genes are located on the chromosomes of A subgenome.