Enhanced transport and antifouling properties of polyethersulfone membranes modified with α-amylase incorporated in chitosan-based polymeric micelles
Loading...
Date
2020
Authors
Kolesnyk, Iryna
Konovalova, Victoria
Kharchenko, Kateryna
Burban, Anatoliy
Kujawa, Joanna
Kujawski, Wojciech
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The objective of this study is related to the modification of polyethersulfone membranes with α-amylase
incorporated into chitosan-based polymeric micelles in order to reduce the membrane fouling. Amphiphilic
chitosan derivatives have been synthesized through grafting of palmitic (PA) and stearic (SA) acids onto low-molecular weight chitosan. CMC values of palmitoyl and stearoyl derivatives of chitosan are equal to
5.7⋅10
2 mg/mL and 3.9⋅10
2 mg/mL, respectively. Whereas, the size of polymeric micelles amounted to
707 -64 nm and 609 - 57 nm for Chit-g-PA and Chit-g-SA, accordingly. The obtained micelles with enzyme have
been adsorbed onto the surface of polyethersulfonic membranes. Modified membranes are characterized by high
stability of the coating over time, thanks to the surface-active properties of chitosan derivatives and electrostatic
interactions. The antifouling biocatalytic properties of modified membranes were studied in the process of starch
filtration. The unmodified membrane showed the lowest permeability with an increase in the concentration of
starch solutions due to dramatically increased cake formation. The permeability of modified membranes has
been improved remarkably compared to the pristine one during starch ultrafiltration. The cake resistance of
modified membranes decreases 6-fold, compared to the unmodified PES one. Furthermore, the effect of concentration polarization is attenuated owing to starch hydrolysis by α-amylase incorporated into polymeric micelles. The maximum activity of α-amylase immobilized on PES membrane is observed in the pH range of 6.5–7.5
and the higher resistance of enzyme to acidic media compared to the native one has been shown. High stability
and reusability of immobilized α-amylase has been demonstrated. The degree of starch conversion reduces by
35% and 30% after 10 cycles for a membrane modified with Chit-g-PA and Chit-g-SA, respectively.
Description
Keywords
α-Amylase, Polyethersulfone membrane, Chitosan, Polymeric micelle, Starch conversion, article
Citation
Enhanced transport and antifouling properties of polyethersulfone membranes modified with α-amylase incorporated in chitosan-based polymeric micelles [electronic resource] / Kolesnyk I., Konovalova V., Kharchenko K., Burban A., Kujawa J., Kujawski W. // Journal of Membrane Science. - 2020. - Vol. 595. - Article number 117605