Detection of biogenic polyamines in blood of patients with breast cancer

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dc.contributor.author Prylutskyi, Maksym
dc.contributor.author Bilko, Nadiia
dc.contributor.author Starodub, Nickolaj
dc.date.accessioned 2020-07-26T13:20:56Z
dc.date.available 2020-07-26T13:20:56Z
dc.date.issued 2019
dc.identifier.citation Prylutskyi M. P. Detection of biogenic polyamines in blood of patients with breast cancer [electronic resource] / Prylutskyi M. P., Bilko N. M., Starodub N. F. // Regulatory Mechanisms in Biosystems. - 2019. - Vol. 10, Issue 2. - P. 257-263. - DOI: 10.15421/021939
dc.identifier.issn 2520-2588
dc.identifier.uri https://doi.org/10.15421/021939
dc.identifier.uri http://ekmair.ukma.edu.ua/handle/123456789/17729
dc.description.abstract The main threat of cancer diseases is their spreading throughout the population of many countries of the world and the complexity of their diagnostics at the early stages. Because of that, search and development of the latest diagnostic methods for oncological diseases which would allow them to be diagnosed more precisely and rapidly is going on. The objective of the study was developing a method for qualitative and quantitative analysis of polyamines as potential tumour markers in blood serum of patients with breast cancer using a newly created immune biosensor based on the effect of surface plasmon resonance (SPR). In the process of the study we used basics of immune analysis, methods of biosensor analysis with preliminary modification of biosensor analytical surface with certain reagents, such as protein A and BSA, for better orientation of the sensitive layer made from antibodies. During the study 30 samples of blood serum were analyzed, 21 of which were obtained from the patients with breast cancer and 9 samples were normal, taken from healthy people and used as controls. Analysis of blood serum samples was made, using previously created calibration curve, based on polyamine solutions in concentrations from 5 ng/mL to 1 µg/mL. It allowed determination of the presence of polyamines in blood samples and approximate concentration of polyamines comparing resonance angle shift in calibration curve and blood samples. According to the obtained results, the concentration of polyamines exceeded their physiological levels and was in the range of 21.3–125.1 ng/mL. The proposed approach allows one to determine the presence and approximate concentrations of polyamines in range from 5 ng/mL to 1 µg/mL in samples of blood serum of patients with breast cancer which correlates with tumour size and the age of the patients. en_US
dc.language.iso en en_US
dc.subject surface plasmon resonance en_US
dc.subject biosensors en_US
dc.subject spermine en_US
dc.subject tumour markers en_US
dc.subject transducer en_US
dc.subject sensogram en_US
dc.subject article en_US
dc.title Detection of biogenic polyamines in blood of patients with breast cancer en_US
dc.type Article en_US
dc.status first published en_US
dc.relation.source Regulatory Mechanisms in Biosystems en_US

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  • Центр молекулярних і клітинних досліджень
    Центр молекулярних і клітинних досліджень створено 2004 року. Стратегічна мета діяльності Центру - сформувати школу клітинної біології та біотехнології, що дасть змогу готувати висококваліфікованих кадрів як для викладання у вищих навчальних закладах, так і для науково-дослідних установ та медико-біологічних центрів, діяльність яких пов'язана з клітинною біологією і спрямована на вирішення проблем, пов'язаних із аналізом, накопиченням і оцінкою клітинних популяцій як трансплантатів.

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