Білковий поліморфізм компонентів схрещування при створенні пшеничних ліній з інтрогресіями від Triticum migushovae Zhir
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Date
2018
Authors
Пасічник, Тетяна
Антонюк, Максим
Терновська, Тамара
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Abstract
Встановити наявність/відсутність полі-морфізму за генами, що кодують білки пшениці з відомою хромосомною локалізацією для ви-значення компонентів схрещування у ході ство-рення інтрогресивних ліній T. aestivum/T. migu-schovae, оптимальних для скринування нащад-ків за досліджуваними білками.
Determine presence/absence of polymorphism at genes coding wheat proteins with known chromosome localization in order to determine cross components for development of introgressive lines T. aestivum/T. miguschovae, which are optimal for screening progeny for studied proteins. Methods. Protein electrophoresis in PAAG, visualization and comparison of spectra. Results. Electrophoretic spectra components which could be used as markers of chromosomes of Ab, G, D genomes of Migushova wheat, and A, B and D genomes of four cultivars of common wheat were identified for 1-st (gliadins, glutenins), 3-rd (leaf and seed esterase, peroxidase), 4-th (beta-amylase, acid phosphatase), 6-th (gliadins, alfa-amylase), 7-th (alfa-amylase) groups of homeological chromosomes. Conclusions. Progeny from any of the four common wheat cultivars can be studied for the presence of Migushova wheat chromosomes that substituted common wheat chromosomes of 1-st, 3-rd, 4-th, 6-th, and 7-th homeological groups, however, effectiveness of studied protein markers varied for different cultivars.
Determine presence/absence of polymorphism at genes coding wheat proteins with known chromosome localization in order to determine cross components for development of introgressive lines T. aestivum/T. miguschovae, which are optimal for screening progeny for studied proteins. Methods. Protein electrophoresis in PAAG, visualization and comparison of spectra. Results. Electrophoretic spectra components which could be used as markers of chromosomes of Ab, G, D genomes of Migushova wheat, and A, B and D genomes of four cultivars of common wheat were identified for 1-st (gliadins, glutenins), 3-rd (leaf and seed esterase, peroxidase), 4-th (beta-amylase, acid phosphatase), 6-th (gliadins, alfa-amylase), 7-th (alfa-amylase) groups of homeological chromosomes. Conclusions. Progeny from any of the four common wheat cultivars can be studied for the presence of Migushova wheat chromosomes that substituted common wheat chromosomes of 1-st, 3-rd, 4-th, 6-th, and 7-th homeological groups, however, effectiveness of studied protein markers varied for different cultivars.
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білковий поліморфізм, схрещування, інтрогресія, Triticum migushovae Zhir, стаття, wheat introgression, Fusarium head blight, Triticum miguschovae, storage proteins, isoenzymes
Citation
Пасічник Т. В. Білковий поліморфізм компонентів схрещування при створенні пшеничних ліній з інтрогресіями від Triticum migushovae Zhir. / Пасічник Т. В., Антонюк М. З., Терновська Т. К. // Фактори експериментальної еволюції організмів. - 2018. - Т. 22. - С. 62-67.